For investigation purposes, RT‐qPCR is typically used not simply to determine the existence or absence of the RNA but to evaluate its concentration. In such cases, a calibration curve need to be A part of every experiment, consisting of a minimum of 6 serial dilutions of an in vitro−transcribed RNA https://www.facebook.com/NewTechFind/posts/pfbid0YPAzGvVz72mKah68ctQg3wP1JFbs9jq9dyoRfz9AkydHS5u7wshhDSMEEcCg7NQCl